| Antibodies |
| mouse anti-Acetylated-α-tubulin |
Sigma/Merck |
T6793 |
| mouse anti-α-tubulin |
Abcam |
ab7291 |
| rabbit anti-active-Caspase3 |
Cell Signaling |
D176 |
| anti-Digoxigenin AP antibody |
Roche |
11093274910 |
| AlexaFlour 405-labeled goat anti-mouse |
Molecular Probes |
A31553 |
| AlexaFlour 555-labeled goat anti-rabbit |
Molecular Probes |
A21428 |
| Bacterial and Virus Strains |
| NEB® 5-alpha Competent E. coli (High Efficiency) |
New England Biolabs |
C2987H |
| Chemicals, Peptides, and Recombinant Proteins |
| Trizol |
Invitrogen |
15596026 |
| RNase Inhibitor |
Promega |
N251B |
| Dig-labeled rNTPs |
Roche |
3359247910, 11277057001 |
| BM Purple |
Roche |
11442074001 |
| NBT/BCIP |
Roche |
11681451001 |
| DMSO |
Roth |
A994.2 |
| DAPT (N-[(3,5-Difluorophenyl)acetyl]-L-al-anyl-2-phenyl]glycine-1,1-dimethylethyl ester |
Sigma-Aldrich |
05942 |
| PTU (Propylthiouracil) |
Sigma-Aldrich |
1578000 |
| Ultrapure water |
ThermoFisher |
10977035 |
| Rapamycin |
Sigma-Aldrich |
37094 |
| Ruxolitinib |
Selleckchem |
S1378 |
| MG132 |
Calbiochem |
474790 |
| MS222 |
Sigma-Aldrich |
E10521 |
| Glutaraldehyde |
Carl Roth |
4157 |
| Cacodylate Buffer |
Science Services |
11650 |
| Osmium tetroxide |
Science Services |
E19150 |
| Uranyl acetate solution |
Science Services |
E22400-1 |
| Ethanol |
Fisher Scientific |
32205 |
| Aceton |
Sigma-Aldrich |
179124 |
| Durcupan resin |
Sigma-Aldrich |
44611 and 44612 |
| Formvar |
Science Services |
E15830-25 |
| Lead Citrate |
Delta Microscopies |
11300 |
| Hexamethyldisilazan |
Carl Roth |
3840.2 |
| CAS Blocking |
ThermoFischer |
00-8120 |
| DAPI |
Invitrogen |
D1306 |
| AlexaFluor 488-labeled Phalloidin |
Molecular Probes |
A12379 |
| AlexaFluor 647-labeled Phalloidin |
Molecular Probes |
A22287 |
| AlexaFluor 647-labeled PNA |
Molecular Probes |
L32460 |
| Critical Commercial Assays |
| M-MLV reverse transcriptase |
Promega |
M3681 |
| PureYield Midiprep kit |
Promega |
A2495 |
| Ambion mMessage Machine kit |
Thermo Fisher |
AM1340 |
| Q5 Site-directed mutagenesis kit |
NEB |
E0554S |
| LysoTracker |
Invitrogen |
L7526 Green DND-26 |
| Terminal Deoxynucleotidyl Transferase |
Invitrogen |
10533065 |
| Terminal Deoxynucleotidyl Transferase |
Roche |
03333574001 |
| T7 RNA polymerase |
Promega |
P2077 |
| SP6 RNA polymerase |
Promega |
P108G |
| Experimental Models: Organisms/Strains |
| Xla.Tg(Xtr.cdknx:GFP)Papal |
EXRC |
RRID:EXRC_0043 |
| wt Xenopus laevis |
EXRC |
N/A |
| Oligonucleotides |
| jag1-ISH-F 3‘-ATGCTCAAAGGTGTGGTGTG-5‘ |
Sigma-Aldrich |
N/A |
| jag1-ISH-R 3‘-CTCTGTGCTGTTTCTAAGTCTC-5‘ |
Sigma-Aldrich |
N/A |
| hes1-ISH-F 3‘-AAAGGATCCATGCCGGCTGATGTGATGGAG-5‘ |
Sigma-Aldrich |
N/A |
| hes1-ISH-R 3‘-AAAGTCGACTTACCAGGGCCTCCAAACAG-5‘ |
Sigma-Aldrich |
N/A |
| dll1-ISH-F 3‘-GATGACTGTGCTTCCTTCC-5‘ |
Sigma-Aldrich |
N/A |
| dll1-ISH-R 3‘-TTGACGTTGAGTAGGCAGAG-5‘ |
Sigma-Aldrich |
N/A |
| dll4-ISH-F 3‘-CACAAGAAATCCCTGCCTG-5‘ |
Sigma-Aldrich |
N/A |
| dll4-ISH-R 3‘-TATACCTCTGTAGCTATCACAC-5‘ |
Sigma-Aldrich |
N/A |
| NICD-BstB1-F 3’-AAATTCGAAAATAAGAAGCGTCGCCGTGAACACG-5’ |
Sigma-Aldrich |
N/A |
| NICD-Sal1-R 3’-AAAGTCGACCTTGAAAGCTTCAGGTATGTGGGTGCG-5’ |
Sigma-Aldrich |
N/A |
| Q5Cla1ATG-F 3’-TATGAATAAGAAGCGTCGCCGT-5’ |
Sigma-Aldrich |
N/A |
| Q5Cla1ATG-R 3’-TCGATTCGATGGGATCCTGCAAAAA-5’ |
Sigma-Aldrich |
N/A |
| Cla1-GFP-F 3’-AAAAAAATCGATATGGTGAGCAAGGGCGAGGAG-5’ |
Sigma-Aldrich |
N/A |
| GFP-Cla1-R 3’-AAAAAAATCGATCTTGTACAGCTCGTCCATGCC-5’ |
Sigma-Aldrich |
N/A |
| BamH1-hLamp1mCherry-F 3‘-AAAAAAGGATCCATGGCGGCCCCCGGCAGCGC-5‘ |
Sigma-Aldrich |
N/A |
| hLamp1mCherry-EcoR1-R 3‘-AAAAAAGAATTCTTACTTGTACAGCTCGTCCATG-5‘ |
Sigma-Aldrich |
N/A |
| pcm1-ISH-F 3’-ATACCATCTACTCTGAAGTTGCCA-5‘ |
Sigma-Aldrich |
N/A |
| pcm1-ISH-R 3’-TTTCATCTTCACCACTGCCA-5‘ |
Sigma-Aldrich |
N/A |
|
pcm1MO 5’-CTTTGTCTCCACAACTTACATGCAG-3‘ |
Gene Tools |
N/A |
| pcm1Spl9-F 3’-CACCAGATAACAGGAGGCA-5’ |
Sigma-Aldrich |
N/A |
| pcm1Spl9-R 3’-ATCAGAAGTCAAGGCGGA-5’ |
Sigma-Aldrich |
N/A |
| Recombinant DNA |
| hLamp1-mCherry pcDNA3.1 |
Addgene |
45147 |
| Software and Algorithms |
| Zeiss Zen Pro Blue |
Zeiss |
N/A |
| Zeiss Zen Black |
Zeiss |
N/A |
| ImageJ/Fiji |
imagej.nih.gov/ij/download.html |
N/A |
| Adobe Photoshop |
Adobe |
N/A |
