Fig. 5.
Supramolecular hydrogel formulation enhances the transfection efficiency of adenovirus in tumor associated myeloid cells. One dose of Adv-GFP, G4/Adv-GFP, SH, or G4/Adv-GFP SH was intratumorally injected into B16 tumors. At 72 h post injection, tumors were collected for evaluation of GFP expression in myeloid cells (a) and leukocytes (b) by flow cytometry. Tumor cell (gated on CD45− cells) viability (%) was evaluated by flow cytometry after Zombie dye staining (c). NS, not significant. *, p <0.05.