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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2021 Jan 21;41(3):e160–e174. doi: 10.1161/ATVBAHA.120.315875

Figure 2. T-cells precede macrophages in the remodeling venous wall in wild type mice.

Figure 2.

(A) Representative photomicrographs showing CD3 (red) and CD68 (green) in the AVF wall at days 0, 3, 7 or 21. Scale bar, 50 μm. (B) Bar graph shows quantification of CD3- or CD68-positive cells in the AVF at day 0, 3, 7 or 21. n=6. P<0.0001 (2-way ANOVA); *P=0.0076 (day 3 vs day 0, post hoc). **P=0.0002 (day 7 vs day 0, post hoc). †P=0.0003 (day 7 vs day 0, post hoc). (C) Representative photomicrographs showing CD4 (green) and IFN-γ (red), CD4 (green) and IL-4 (red) or CD4 (green) and Foxp3 (red) in the AVF wall at days 0, 3, 7 or 21. Scale bar, 50 μm. (D) Bar graph shows quantification of CD4+IFN-γ+ cells in the AVF at day 0, 3, 7 or 21. n=3. P<0.0001 (ANOVA), P=0.0017 (Kruskal-Wallis); **P<0.0001 (day 3 vs day 0, post hoc). *P=0.0027 (day 7 vs day 0, post hoc). (E) Bar graph shows quantification of CD4+IL-4+ cells in the AVF at day 0, 3, 7 or 21. n=3. P<0.0001 (ANOVA), P<0.0001 (Kruskal-Wallis); **P<0.0001 (day 3 vs day 0, post hoc). (F) Bar graph shows quantification of CD4+Foxp3+ cells in the AVF at day 0, 3, 7 or 21. n=3. P<0.0001 (ANOVA), P=0.0012 (Kruskal-Wallis); **P<0.0001 (day 3 vs day 0, post hoc). *P=0.0386 (day 7 vs day 0, post hoc).