Skip to main content
. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2021 Jan 21;41(3):e160–e174. doi: 10.1161/ATVBAHA.120.315875

Figure 7. Effects of CsA on AVF wall thickening and adaptive remodeling are lost in nude mice.

Figure 7.

(A) Representative photomicrographs of the AVF wall (days 7 and 21) in mice treated with vehicle (control) or cyclosporine (CsA); upper panels, Elastin van Gieson (EVG); middle panels, Masson trichrome stain; lower panels, picrosirius red stain. Scale bar, 25 μm. (B) Bar graph shows intima-media thickness of the AVF wall in mice treated with control or CsA. n=4–5. P=0.6575 (ANOVA). Day 7, P=0.6666 (post hoc). Day 21, P=0.2385 (post hoc) (C) Bar graph shows relative collagen density in the Masson trichrome stained AVF wall in nude mice treated with control or CsA, normalized to the control group (day 7). n=4–5. P=0.7690 (ANOVA). Day 7, P=0.8680 (post hoc). Day 21, P=0.6619 (post hoc). (D) Bar graph shows relative collagen density in the picrosirius red stained AVF wall in nude mice treated with control or CsA, normalized to the control group (day 7). n=4–5. P=7132 (ANOVA). Day 7, P=0.7994 (post hoc). Day 21, P=0.9988 (post hoc). (E) Representative photomicrographs of the AVF wall (days 7 and 21) in nude mice treated with control or CsA stained with anti-collagen I antibody. Scale bar, 25 μm. Bar graph shows relative collagen I density in the AVF wall, normalized to the control group (day 7). n=5. P=0.8608 (ANOVA). (F) Line graph shows relative AVF diameter of nude mice treated with vehicle (control) or CsA, normalized to day 0. n=10. P=0.8241 (group factor, ANOVA). Day 3, P=0.6643 (post hoc). Day 7, P=0.9732 (post hoc). Day 14, P=0.8424 (post hoc). Day 21, P=0.9996 (post hoc).