| Antibodies |
|
|
| Mouse α-Cdc27 |
BD Biosciences |
#610455, RRID:AB_397828 |
| mouse α-cyclin B2 |
Santa Cruz Biotechnology |
#sc-53239, RRID:AB_629356 |
| rabbit α-Nup53 serum |
85 |
|
| rabbit α-PPP1A pT320 |
Abcam |
#ab62334, RRID:AB_956236 |
| rabbit α-Cdc25C |
44 |
|
| rabbit α-Wee1 pT150 |
86 |
|
| rabbit α-Greatwall serum |
60 |
|
| rabbit α-ENSA serum |
60 |
|
| rabbit α-Arpp19 |
63 |
|
| rabbit α-Cdk1 pY15 |
Cell Signaling Technology |
#9111L, RRID:AB_331460 |
| rabbit α-ENSA pS67/Arpp19 pS62 |
Cell Signaling Technology |
#5240, RRID:AB_11220425 |
| mouse α-FLAG M2 |
Sigma-Aldrich |
#F1804, RRID:AB_262044 |
| peroxidase-linked sheep α-mouse IgG |
GE Healthcare |
#NA931V, RRID:AB_772210 |
| AMDEX goat α-rabbit IgG |
GE Healthcare |
RPN4301, RRID:AB_2650489 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| PD0166285 |
Abcam |
ab219507 |
| Okadaic acid |
EMD Millipore |
#495609 |
| 10% Criterion Tris-HCl precast gel |
Biorad |
#3450011 |
| 4–20% Criterion Tris-HCl precast gel |
Biorad |
#3450034 |
| Phos-tag |
NARD Institute |
AAL-107 |
| Human chorionic gonadotropin |
Merck |
Chorulon |
| Human chorionic gonadotropin |
Sigma-Aldrich |
#CG10-10VL |
| A23187 |
Sigma-Aldrich |
# C7522 |
| Histone H1 |
Millipore |
#14-155 |
| PKA inhibitor IV |
Santa Cruz Biotechnology |
#sc-3010 |
| FLUOTRAC 200 384 well plates |
Greiner |
#781076 |
| Cyclin A2/CDK2 complex |
Sigma |
#C0495 |
| Protease inhibitor cOmplete |
Roche |
#11873580001 |
| Magnetic Protein G beads |
Invitrogen |
#10004D |
| Critical Commercial Assays |
|
|
| TNT SP6 high-yield wheat germ protein expression system |
Promega |
#L3261 |
| mMESSAGE mMACHINE kit |
Invitrogen |
#AM1344 |
| Poly(A) tailing kit |
Invitrogen |
#AM1350 |
| MEGAclear Transcription Clean-Up kit |
Invitrogen |
#AM1908 |
| Experimental Models: Organisms/Strains |
|
|
| Oocyte plus female Xenopus laevis
|
Nasco |
LM00531 |
| Recombinant DNA |
|
|
| pQE80-6His-Arpp19 |
63 |
|
| pQE80-6His-Arpp19 S28A S109A |
63 |
|
| pCS2-T7-Flag3-Gwl K71M |
63 |
|
| pMal-c5x-fizzy 38-62 aa |
This study |
|
| Software and Algorithms |
|
|
| Mathematica 10 and 12 |
Wolfram Research |
https://www.wolfram.com/mathematica/ |
| Graphpad Prism 8.0.2 and 8.4 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
| Fiji (ImageJ, version 2.00-rc-49/1.51e) |
87 |
https://imagej.net/Fiji |
| Excel |
Microsoft |
N/A |
| Python 3.7.3 |
88 |
https://www.python.org/ |
| Other |
|
|
| Flexstation II |
Molecular Devices |
N/A |
| Phosphorimager Typhoon 8600 |
Molecular Devices |
N/A |
