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. 2021 Feb 11;12:632280. doi: 10.3389/fmicb.2021.632280

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Copyright © 2021 Bolaji, Wan, Manchur, Lawley, de Kievit, Fernando and Belmonte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of crop sequence on fungal species diversity in the bulk soil Principal coordinate analysis (PCoA), represented by the beta diversity metric – Weighted UniFrac, comparing the effect of crop sequence on microbial species diversity among treatments at all time points in the bulk soil. For each axis, the percent of variation is indicated. Dark blue dots, canola-soybean sequence (Ca-S); light blue dots, soybean-soybean sequence (S-S); purple dots, corn-soybean sequence (C-S). Each dot represents the average of three biological replicates and each biological replicate represents five pooled core soil samples. Comparison of cumulative fungal species diversity for all sequences collect at (A) all time-points (B) pre-seeding (C) V6, and (D) R1 soybean growth stages. Because of similarities in the data, some data points are overlapping.