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. 2021 Feb 11;12:632280. doi: 10.3389/fmicb.2021.632280

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Copyright © 2021 Bolaji, Wan, Manchur, Lawley, de Kievit, Fernando and Belmonte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Principal coordinate analysis (PCoA), represented by Bray-Curtis dissimilarities between samples and calculated using the 16S rRNA amplicon sequences for bacterial samples and the ITS1 and ITS2 amplicon sequences of fungal samples. Soils were obtained from the Ca-S, S-S, and C-S crop sequences in the bulk and rhizosphere soil for all time-points. For each axis, the percent of variation is indicated. The dark blue filled and open circles represent the canola-soybean sequence (Ca-S), light blue filled and open circles are the soybean-soybean sequence (S-S) and the purple filled and open circles are the corn-soybean sequence (C-S). Each dot represents the average of three biological replicates and each biological replicate represents five pooled core soil samples. Comparison of cumulative Bray-Curtis dissimilarities for panel (A) bacterial in the bulk soil (B) bacterial in the rhizosphere soil (C) fungal in the bulk soil (D) fungal in the rhizosphere soil.