Fig. 9.

Camel required for the development of SCO and formation of Reissner fiber. a, b Control. c, d Pan-anti-Camel morphants (MO1). All images are of ET27 transgenics at 48 hpf. a, c In vivo images of GFP expression. b, d Double immunostaining for GFP and Reissner fiber. b’, d’ Blowup of boxed areas in b and d, respectively. b’ The AFRU staining in controls is mostly presented by the filamentous extracellular material with gaps between SCO and AFRU material (arrow) and in the AFRU material (arrowhead). Some faint AFRU staining was detected in the cytoplasm (asterisk). d’ In morphants, the AFRU material is disorganized; it covers the SCO more with some residual signal in the cytoplasm (asterisk). Scale bar = 100 μm (a–d) and 50 μm (b’, d’)