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. 2021 Feb 24;11:4504. doi: 10.1038/s41598-021-83888-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Boxplot of score distribution according to pretreatment of formalin-fixed paraffin-embedded tissues. Choice of pretreatment for permeabilization: Heat-induced microRNA Retrieval in Target Retrieval Solution (TRS) pH 6.1, TRS pH 9 or proteolysis with proteinase K. Exposure time for proteolysis treatment is given in minutes and presented in (). All scores are given regardless of tissue type, hence revealing the overall score of probe-staining effectivity. The x-axis shows type of treatment. The y-axis shows the mean score of the probe staining on a scale from 0 to 3, where 0 = no staining of the probe, and 3 is strong staining of the probe. The darker the color, the better the staining. The figure was constructed with the statistical software R.