Fig. 3. Cathelicidin induces IL-17F-producing but not IL-22-producing T cells.

Splenocytes isolated from C57BL/6 J mice were cultured in Th17 conditions for 48 h in the presence or absence of 2.5 μM cathelicidin. A–D Expression of IL-17A and F was determined by intracellular flow cytometry. E IL-17F and F IL-22 production was quantified by ELISA of cell culture supernatant after 48 h in culture. Data shown are individual mice in separate experiments. B–F are analysed by paired two-tailed t-tests. SP = single positive. N values: B - 9, C - 9, D - 9, E - 5, F - 4. Black symbols represent untreated samples and open symbols are samples treated with cathelicidin.