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. 2021 Feb 24;12:1285. doi: 10.1038/s41467-021-21533-5

Fig. 7. Mice lacking cathelicidin cannot increase IL-17A production in response to inflammation.

Fig. 7

Wild-type (WT) C57BL/6 J and fully backcrossed cathelicidin knockout (Camp−/−, KO) mice were culled and A, B organs removed and IL-17A production assessed ex vivo by flow cytometry. C Mice were inoculated with 12.5 μg/ml heat-killed Salmonella typhimurium and draining lymph nodes removed seven days later—IL-17A was measured by flow cytometry. D Splenic T cells from WT or KO mice were stimulated in Th17-driving conditions for 48 h and IL-17A quantified by flow cytometry. E–G WT and KO mice were injected intravenously with 5 million OT-II T cells and 24 h given ovalbumin in complete Freund’s adjuvant subcutaneously. On day 7 spleens and lymph nodes were removed and donor cell survival and IL-17A production assessed by flow cytometry. Data shown are individual mice with line at median. C–G were analysed with two-tailed t-tests with no correction. PP—Peyer’s Patches; mLN = mesenteric lymph node. N values: B - 4, C - 3, D - 4, E - 4, F - 4, G - 4. Black symbols represent wild-type mice and open symbols represent mice lacking cathelicidin.