Fig. 2. H₂O₂ induction modulates mitophagy-associated protein levels.

A Cells were transfected with the EGFP-C1 (as a control) or EGFP-Parkin plasmids. Twenty-four hours later, the cells were treated with 100 μΜ H₂O₂ for 0 h, 6 h, 12 h, or 18 h. The levels of the mitophagy-associated proteins OPTN, NDP52, and p62 were evaluated by WB analysis. N = 4; ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Data from four independent tests were collected for statistical analysis (mean ± SD). B Cells were transfected with the EGFP-C1 or EGFP-Parkin plasmids. Twenty-four hours later, the cells were treated with 100 μΜ H₂O₂ for 0 h, 6 h, 12 h, or 18 h. The levels of MFN2 and MFN1 were evaluated by WB analysis. N = 3; *P < 0.05; ***P < 0.001; ****P < 0.0001. Data from three independent tests were collected for statistical analysis (mean ± SD).