Fig. 4. c-Myc is upregulated in H₂O₂-induced cells.

A, C, D EGFP-Parkin-transfected and EGFP-C1-transfected HeLa cells were stimulated with 100 μΜ H₂O₂ for 0 h or 12 h and stained with anti-Myc, anti-Myc-S⁶², and anti-Myc-T⁵⁸ antibodies (red). Scale bars, 10 μm. B, E, F Cells were transfected with EGFP-C1 or EGFP-Parkin plasmids. After 24 h, the cells were treated with 100 μΜ H₂O₂ for 0 h or 12 h. Nuclear extracts were immunoblotted for c-Myc, p-c-Myc-S62, and p-c-Myc-T58. Lamin B1 was used as a nuclear internal reference, and Tubulin was used as a cytoplasmic internal reference. N = 3; *P < 0.05; **P < 0.01. The data are from three independent tests and are presented as the mean ± SD.