Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 24;11:4480. doi: 10.1038/s41598-021-83979-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Transient knockdown of TKT with siRNA augments oxidative stress levels, but does not increase sensitivity to Erastin in H1299 p53^null cells. (A) Concentration of NADPH, normalised to total protein concentration, in H1299 parental (p53^null) cells following 18 h APR-246 (25 µM) treatment administered after 72 h TKT knockdown with siRNA (siNTC, non-targeting control; siTKT, TKT siRNA). (B) Geometric mean fluorescence intensity of MitoSOX Red in H1299 parental (p53^null) cells following 18 h APR-246 (25 µM) treatment administered after 72 h TKT knockdown with siRNA, measured with flow cytometry (siNTC, non-targeting control; siTKT, TKT siRNA). (C) Cell viability in H1299 parental (p53^null) cells following 72 h APR-246 (15 µM) treatment and/or N-acetyl-cysteine (NAC, 5 mM) supplementation, following 24 h TKT knockdown with siRNA, shown as a percentage of vehicle control. (D) Cell viability (AlamarBlue) in H1299 cells following 72 h treatment with 1 or 2.5 µM of Erastin, administered 24 h after TKT knockdown with siRNA, normalised to siNTC vehicle control (siNTC, non-targeting control; siTKT, TKT siRNA). Data represent mean, error bars = SEM, n = 3 for all studies. (A,B,C) One-way ANOVA with Dunnett’s multiple comparison post-test. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.