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. 2021 Feb 24;12:1269. doi: 10.1038/s41467-021-21247-8

Fig. 5. ATRX/DAXX complex abundance.

Fig. 5

a ATRX protein and ATRX mRNA expression in ALT ATRX-mutated (dark pink) and wild-type (light pink) tumors compared to TERT (blue), MNA (yellow), and OTHER (gray). b Boxplots of DAXX protein and DAXX mRNA expression in ALT ATRX-mutated (dark pink) and wild-type (light pink) tumors compared to TERT (blue), MNA (yellow), and OTHER (gray). a, b Boxplots indicate the median value (middle line) and the 25th and 75th percentiles (box). The upper/lower whisker spans from the hinge to the largest/smallest value (values expanding a distance of 1.5 x inter quartile range are not considered). Dots represent individual tumors. P values in boxplots were calculated with two-sided Wilcoxon rank sum tests. n describes the number of analyzed tumors. c, d ATRX protein expression in NBL-S cells (with moderate MYCN expression) after 96 h treatment with siRNAs against DAXX (black). ATRX siRNAs as well as two negative control siRNAs were used as controls (gray). Exemplary western blot (c) and quantification of three biological replicates (d) is shown. P values calculated using a two-sided Student’s t-test. Horizontal line shows median value. Dots represent biological replicates. Uncut images of all biological replicates are shown in the source data file.