Table 2.
Platform comparison.
| Technology | miRNA-Seq | nCounter | qPCR | |||
|---|---|---|---|---|---|---|
| Platform | Illumina TruSeq | NanoString | Qiagen miScript | Exiqon miRCURY | ABI Taqman | MiRXES ID3EAL |
| Panel content (number of miRNAs) | Up to 2588a | 800 | 1066 | 751 | 733 | 560 |
| Turnaround time (days)b | 4 | 2 | 7 | 5 | 5 | 7 |
| Reagent cost per sample | + | + | ++ | ++ | ++ | ++ |
| Concordance correlation coefficientc | 0.99 | 0.82 | 0.91 | 0.95 | 0.96 | 0.99 |
| microRNAs detected above LLOQ | 372d | 84 | NA | 208 | 179 | 438 |
aNumber of mature microRNAs in miRBase release 21.
bTo obtain expression data for 12 samples using one instrument.
cAverage of pairwise comparisons across a minimum of three independent runs.
dBased on sequencing depth of 20 million reads.