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. 2021 Feb 24;11:4494. doi: 10.1038/s41598-020-80425-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mice presented improved exploratory behaviors under SHH conditions. (a) Scheme of the elevated plus maze test after 16 h of SHH5000 or SHH8000 treatment aiming to examine the exploratory behaviors of mice. (b) Trajectory pattern diagram after SHH5000 or SHH8000 treatment. (c) Total arms times, total distance and speed (from left to right). (d) Closed arm entry times, movement distance in the closed arm and residence time in the closed arm (from left to right). (e) Open arm entry times, movement distance in the open arm and residence time in the open arm (from left to right). (f) Central entry times, central distance and central residence time (from left to right). Each value is expressed as the mean ± standard error of the mean. Multiple comparisons between groups were carried out with Tukey’s post hoc test. *P value < 0.05; **P value < 0.01; ***P value < 0.001, n = 5 per group.