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. 2021 Feb 24;11:4494. doi: 10.1038/s41598-020-80425-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In the cerebellum of mice with simulated hypobaric hypoxia treatment, only the levels of Mfn2 and UCP4 were statistically increased by SHH5000, whereas all other genes were almost the same as those in the normal control group. (a) Normal mice were sacrificed at SHH5000 or SHH8000 for 16 h. The mice were sacrificed and the cerebellum was collected, immediately stored in liquid nitrogen. Real-time quantitative PCR was then performed to detect mitochondrial-related molecules (Drp1, Mfn1, Mfn2, Opa1, TFAM, SGK1, UCP2, UCP4) mRNA expression levels differences. (b) Simulated hypobaric hypoxia induced mitochondrial dynamic altering in cerebellum of mice. qRT-PCR analyses of Drp1, Mfn1, Mfn2, Opa1, TFAM, SGK1, UCP2, UCP4 in cerebellum of mice. In the cerebellum of mice with SHH treatment, only the levels of Mfn2 and UCP4 were statistically increased by SHH5000, whereas all other genes, including DRP1, other mfn1 and Opa1, TFAM, SGK1 and UCP2 were almost the same as those in the normal control group. Data are presented as mean SD. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group. n = 5 per group. (c) Schematic depiction of the mitochondrial dynamics within the cerebellum of mice in different conditions including normal control, exposed at SHH5000 and SHH8000, respectively. It can be found the mRNAs levels of six mitochondria-related genes including Drp1, Mfn1, Opa1, SGK1, TFAM and UCP2 remained stable except that the mRNAs levels of Mfn2 and UCP4 increased significantly only at SHH5000. Drp1, dynamin-related protein 1, mitochondrial fission factor on the mitochondrial outer membrane; Mfn1, mitochondrial fusion protein 1, mitochondrial fusion factor on the mitochondrial outer membrane; Mfn2, mitochondrial fusion protein 2, mitochondrial fusion factor on the mitochondrial outer membrane; Opa1, Optic atrophy 1, mitochondrial fusion factor on the mitochondrial inner membrane; SGK1, serum and glucocorticoid-regulated protein kinase 1, mitophagy-related factor; TFAM, mitochondrial transcription factor; UCP2, uncoupling protein 2, mitochondrial stress factor; UCP4, uncoupling protein 4, mitochondrial stress factor; SHH5000, simulated hypobaric hypoxia at altitude 5000 m; SHH8000, simulated hypobaric hypoxia at altitude 8000 m.