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. 2021 Feb 24;11:4494. doi: 10.1038/s41598-020-80425-8

Figure 5.

Figure 5

In the hippocampus, no changes in any gene mRNA levels were detected after SHH5000 and SHH8000 treatment. (a) Normal mice were sacrificed after SHH5000 or SHH8000 treatment for 16 h. The mice were sacrificed, and each hippocampus was collected and immediately stored in liquid nitrogen. Real-time quantitative PCR was then performed to detect differences in the mRNA expression levels of mitochondrial-related genes (Drp1, Mfn1, Mfn2, Opa1, TFAM, SGK1, UCP2, UCP4). (b) Simulated hypobaric hypoxia-induced mitochondrial dynamic alterations in the hippocampus of mice. qRT-PCR analyses of Drp1, Mfn1, Mfn2, Opa1, TFAM, SGK1, UCP2, and UCP4 in the hippocampus of mice. In the hippocampus of mice after SHH treatment, no changes in the mRNA levels of any of the genes examined were detected. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group. n = 5 per group. (c) Schematic depiction of the mitochondrial dynamics within the hippocampus of mice after different treatments, including the normal control and after to SHH5000 and SHH8000 treatment. Drp1, dynamin-related protein 1, a mitochondrial fission factor on the mitochondrial outer membrane; Mfn1, mitochondrial fusion protein 1, a mitochondrial fusion factor on the mitochondrial outer membrane; Mfn2, mitochondrial fusion protein 2, a mitochondrial fusion factor on the mitochondrial outer membrane; Opa1, Optic atrophy1, a mitochondrial fusion factor on the mitochondrial inner membrane; SGK1, serum and glucocorticoid-regulated protein kinase 1, a mitophagy-related factor; TFAM, mitochondrial transcription factor; UCP2, uncoupling protein 2, a mitochondrial stress factor; UCP4, uncoupling protein 4, a mitochondrial stress factor; SHH5000, simulated hypobaric hypoxia at an altitude of 5000 m; SHH8000, simulated hypobaric hypoxia at an altitude of 8000 m.