Table 1.
Sequences of the primers employed for amplification of mRNAs encoding Drp1, Mfn1, Mfn2, Opa1, TFAM, SGK1, UCP2 and UCP4 by qRT-PCR.
| mRNA | Sequences (5′–3′) | Length | Annealing temperature (°C) | |
|---|---|---|---|---|
| Drp1 |
Forward: Reverse: |
5′-AACAGGCAACTGGAGAGGAA-3′ 5′-GCAACTGGAACTGGCACAT-3′ |
144 | 58 |
| Mfn1 |
Forward: Reverse: |
5′-GGTCTGCTTTCCTGCTCTCT-3′ 5′-CTTTCTGCTCCCATTTCACC-3′ |
117 | 60 |
| Mfn2 |
Forward: Reverse: |
5′-CCTGGGATCGATGTTACCAC-3′ 5′-AACTGCTTCTCCGTCTGCAT-3′ |
119 | 62 |
| Opa1 |
Forward: Reverse: |
5′-GCCTTCCTCTTCGTCTCTCC-3′ 5′-CTCACTTGCTTCCACACCAA-3′ |
111 | 60 |
| TFAM |
Forward: Reverse: |
5′-GCAGGCACTACAGCGATACA-3′ 5′-TACCTTTCCCATTCCCTTCC-3′ |
128 | 58 |
| SGK1 |
Forward: Reverse: |
5′-GGTGGACTGGTGGTGTCTTG-3′ 5′-GAAGGAATCCACAGGAGGTGC-3′ |
122 | 62 |
| UCP2 |
Forward: Reverse: |
5′-TGGGAGGTAGCAGGAAATCA-3′ 5′-GCGGTATCCAGAGGGAAAGT-3′ |
135 | 60 |
| UCP4 |
Forward: Reverse: |
5′-CTCAGAGCCAACCGAATAGC-3′ 5′-GGCTGACAGATGCAACAGAA-3′ |
142 | 58 |