Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 24;12(2):211. doi: 10.1038/s41419-021-03493-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — BRL-3A cells transfected with TRAF3 Lys138 mutants (K138R) plasmids or the corresponding WT and vector plasmids as indicated, and the cells were cultured under normal or H/R conditions. The following analysises were performed. A–C ALT, AST, and LDH activities in the culture medium. Pretreated BRL-3A cells were incubated under normal or H/R conditions. Data are mean ± SD, n.s., not significant; **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 3 per group. D CCK-8 assay was used to examine cell survival. Data are mean ± SD, n.s., not significant; **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 6 per group. E ROS in BRL-3A cells. ROS staining was performed to evaluate the level of ROS in BRL-3A cells, and the stained fluorescence was observed using a fluorescence microscope. The cellular morphology and structure of BRL-3A cells by bright image investigation. F–J Proteins levels of p-IKKβ, IKKβ, p-IκBα, IκBα, p-p65, p65 in BRL-3A cells were evaluated using western blot (F) and quantified using a Gel-Pro Analyzer (G–J). Data are mean ± SD, n.s., not significant; **P < 0.01 by two-way ANOVA followed by Tukey’s test. n = 3 per group.