Fig. 8. Spatially progressive epigenetic remodeling in ischemic injury.

a Volcano plot of Z-scored transcription factor (TF) motif enrichment slope change across punch positions 5–8 (Δslope = slope_stroke − slope_{contralateral}) by −log10 p value of the two-way ANOVA from the interaction of TF motif enrichment per punch by condition (stroke, contralateral) without multiple comparison correction. Colored by significance (N.S. not significant, Sig. Δ slope = significant change in slope, Sig. Δ Slope and p = significant change in slope and significant p value). b Top hits for significantly different changes in TF motif enrichment over space as compared between stroke (red) and contralateral (blue); KLF9 (top) and BHLHA15 (bottom). −Log10 p value of the two-way ANOVA from the interaction of TF motif enrichment per punch by condition without multiple comparison correction. Data are presented as linear fitted model ± SEM. c KLF9 TF motif enrichment over space reveals cell type contribution to KLF9 enrichment from infarct core to peri-infarct area. Cell types as defined in Fig. 7d. d Comparison of TF motif enrichment at the infarct border (punch position 8) between stroke (punch 40) and contralateral (punch 48) single-cell profiles. Oligodendrocyte (Olig) TF motif enrichment shown for BCL11B and RXRG for punch 40 (n = 4 cells) and punch 48 (n = 6 cells). Glutamatergic neuron (Glut) TF motif enrichment shown for KLF4 and KLF7 for punch 40 (n = 5 cells) and punch 48 (n = 3 cells). Two-sided Mann–Whitney U test with Bonferroni–Holm correction. Center line represents median, lower and upper hinges represent first and third quartiles, whiskers extend from hinge to ±1.5 × IQR, individual cells represented as colored dots. Source data are provided as a Source data file.