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. 2021 Feb 24;12(2):210. doi: 10.1038/s41419-021-03490-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Electron micrograph of the exosomes extracted from ascites of patient #2. Scale bar, 200 nm. b Western blot analysis of CD63, CD9 and calnexin in ADEs #2 and #3 as well as in the cell pellet control. c Size analysis of the ADEs #2 and #3 using NTA. d Size analysis of ADEs #2 and #3 using DLS analysis. e Flow cytometric analysis of the expression of CD63 and CD9 in ADEs #2 and #3. f Immunofluorescence detection of ADE uptake by SKOV3 cells. In exosomes, PKH67 was labeled with green, and F-actin was labeled red; nuclei were labeled with DAPI (blue). Scale bar, 20 μm.