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. 2021 Feb 24;12(2):210. doi: 10.1038/s41419-021-03490-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Primary tumor cells extracted from two patients were treated with 0 μg, 50 μg, or 100 μg of #7 ADEs. a Representative images of morphological changes in primary tumor cells. Scale bar, 100 μm. b Representative images of in vitro transwell migration assays of primary tumor cells. Scale bar, 100 μm. c The numbers of migrated cells in transwell migration assays were counted and are shown as histograms. d Representative images of in vitro wound-healing assays of primary tumor cells. Scale bar, 200 μm. e The areas of wound closure were measured and are shown as histograms. f Representative images of in vitro colony formation assays of primary tumor cells. g The percentages of cells forming colonies are shown as histograms. h Western blot analysis of the changes in the expression of EMT markers in primary tumor cells. Experiments were performed in at least triplicate, and the results are shown as the mean ± s.d. values. Student’s t test was used to analyze the data (NS, not significant; *p < 0.05; **p < 0.01; ***p < 0.001).