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. 2021 Feb 24;12(2):210. doi: 10.1038/s41419-021-03490-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a qRT-PCR was used to compare miR-6780b-5p expression among 18 ADEs with peritoneal metastasis and nine without peritoneal metastasis. ★, #7 ADEs. b Kaplan–Meier analysis of miR-6780b in pancancer analysis with K-M Plotter. c qRT-PCR was used to compare miR-6780b-5p expression in four ovarian cancer cell lines. d qRT-PCR was used to compare the relative miR-6780b-5p expression in four sets of ADEs (#6, #12, #21, #25) from four HGSOC patients. e Western blots of E-cadherin, N-cadherin and Vimentin expression in A2780, CAOV3, ES2, and SKOV3 cells treated with four sets of ADEs with different miR-6780b-5p concentrations. f qPCR validation of the effects of the miR-6780b-5p agomir and antagomir in A2780, CAOV3, ES2, and SKOV3 cells. g Representative images of in vitro wound-healing and transwell migration assays with SKOV3 and ES2 ovarian cancer cells after transfection of miR-6780b-5p stable NC/agomir and inhibitor NC/antagomir. Scale bar for the wound-healing assay, 100 μm. Scale bar for the transwell assay, 100 μm. h The areas of wound closure and the numbers of migrating cells in transwell migration assays were measured and are shown as histograms. i Western blot analysis of the expression of E-cadherin, N-cadherin, and Vimentin in A2780, CAOV3, ES2, and SKOV3 cells after transfection with miR-6780b-5p agomir and antagomir. Experiments were performed in at least triplicate, and the results are shown as the mean ± s.d. values. Student’s t test was used to analyze the data (NS, not significant; *p < 0.05; **p < 0.01; ***p < 0.001).