Fig. 2.

Generation of CD105-chimeric antigen receptor T cells. a Schematic illustration of vector expressing CARs, CAR with the signal peptide, anti-CD105 Nanobody (C184), CD8α hige and transmembrane domain, 4-1BB costimulatory endodomain, and CD3ζ signaling domain along with GFP using IRES. b HDR-mediated integration of CAR into the AAVS1 locus. Top, donor containing the CAR, CRISPR/Cas9-targeted CAR gene integration into the AAVS1 locus cassette flanked by homology arms. c Expression of GFP by fluorescence microscope after electroporation into T cells. d CD105-specific CAR expression on human T cells were analyzed using flow cytometry