Fig. 3.

CD105 CAR-T cells undergo activation, antigen-induced differentiation, and proliferation. a Anti-CD105 CAR, CD19 CAR, Mock, and the Utd T cells were incubated with CD105⁺ target cells for 3 days; their activation and memory markers were analyzed by flow cytometry. Quantitative analysis of the frequency of activated and memory T cells. b, c T cells were labeled with PKH26 and incubated with CD105⁺ target cell for 5 days. The proliferation of CD105 CAR-T cells was analyzed by flow cytometry. d T cells were labeled with PKH26 and incubated with CD105⁺ cells. The T cells were sampled and counted every other day. Data are present as the mean ± SD from three separate experiments, The quantitative analysis was used by one-way ANOVA with multiple comparisons test