Fig. 7.

Treatment with anti-CD105 CAR-T cells increased T-cell tumor infiltration, reduced the CD105 expression in the tumor tissues, and increased tumor cells apoptosis. a Tumors were collected from mice bearing Bel7404 subcutaneous xenografts treated with CD105 CAR, CD19 CAR, Mock, Utd, or PBS. Formalin-fixed, paraffin-embedded tumor sections were consecutively cut and stained for human CD3 expression, CD105 expression, and TUNEL assays. b Quantitative analysis of T-cell infiltrates in tumor tissues. c Quantitative analysis of the CD105 expression. d Quantitative analysis of apoptotic cells. Data are representative images (magnification ×100) or expressed as the mean ± SD from five randomly selected fields of tumor thin sections. The quantitative analysis was used by one-way ANOVA with multiple comparisons test