Skip to main content
. 2021 Feb 24;12:1278. doi: 10.1038/s41467-021-21529-1

Fig. 6. The 7SK complex regulates Smn complex activity.

Fig. 6

a Western blot analysis of proteins co-immunoprecipitated by anti-Smn from NSC-34 cells treated with DMSO or ActD for 6 h. b Co-immunoprecipitation of Larp7 by anti-Smn from subcellular fractions of NSC-34 cells treated with DMSO or ActD for 1 h. Cyt, cytosol; Nuc, nuclear soluble proteins and organelles; Chr, chromatin-associated proteins. c qPCR analysis of 7SK co-precipitated by anti-SmB/B’ from NSC-34 cells treated with DMSO or ActD for 6 h. Data are mean ± s.d.; ****P ≤ 0.0001; unpaired two-tailed t-test (n = 3). d qPCR analysis of 7SK and snRNAs co-precipitated by anti-Smn from NSC-34 cells treated with DMSO or ActD for 6 h. Data are mean ± s.d.; *P ≤ 0.05; n.s., not significant; two-way ANOVA with Tukey’s multiple-comparisons test (n = 3). e Western blot analysis of Larp7/Mepce double-knockdown (shLarp7-shMepce) or control NSC-34 cells treated with DMSO or ActD for 6 h. f Quantification of Western blot signals in e. Data are mean ± s.d.; ****P ≤ 0.0001; n.s., not significant; two-way ANOVA with Tukey’s multiple-comparisons test (n = 3). g qPCR analysis of U1 and U2 snRNA co-precipitated by anti-SmB/B’ from Larp7/Mepce double-knockdown or control NSC-34 cells treated with DMSO or ActD for 6 h. Data are mean ± s.d.; *P ≤ 0.05; n.s., not significant; two-way ANOVA with Tukey’s multiple-comparisons test (n = 6). h Co-immunoprecipitation of Smn by anti-Larp7 from brains of mice aged E18, P2, P17, and 3 months. i Quantification of Western blot signals in h. Data are mean ± s.d.; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; one-way ANOVA with Tukey’s multiple-comparisons test (n = 4). j qPCR analysis of 7SK RNA co-precipitated by anti-Smn from mouse brains. Data are mean ± s.d.; *P ≤ 0.05; one-way ANOVA with Tukey’s multiple-comparisons test (n = 3). Source data are provided as a Source Data file.