Table 1.
Affinity of different anti-CD2 antibodies for CD2 and Fc gamma receptor (FcγR) I, IIA and IIIA.
| KD (a) | Siplizumab | DG(b) siplizumab | FcS(c) IgG1 | FcS IgG2 | FcS IgG4 |
|---|---|---|---|---|---|
| CD2 | 1.2 | 1.2 | 1.5 | 0.9 | 1.4 |
| Fc gamma RI | 0.2 | 1.13 | >2000 | >2000 | >2000 |
| Fc gamma RIIA | 579 | >5000 | >5000 | >5000 | >5000 |
| Fc gamma RIIIA | 131 | >2000 | >5000 | >5000 | >5000 |
KD of siplizumab, DG siplizumab, FcS anti-CD2 IgG1, FcS anti-CD2 IgG2, and FcS anti-CD2 IgG4 for CD2, FcγRI, FcγRIIA, and FcγRIIIA was measured via surface plasmon resonance.
(a)KD, Affinity constant; (b)DG, deglycosylated; (c)FcS, Fc-silent.