FIGURE 5.

Impact of PCSK9 on CTPR9-induced signal transduction. Adult rat cardiomyocytes were incubated in the presence of LacZ-containing supernatant (control), murine wild-type PCSK9 or GOF PCSK9 (each 0.5 μg/ml) in serum-free medium for 24 h and subsequently treated with CTRP9 (4 μg/ml) for 20 min. Activation of AMPK and Akt was analyzed by Western Blotting. Representative Western blots and densitometry of protein data are shown. Total AMPK and Akt as well as GAPDH served as a loading control. All data are mean ± SEM, 4 independent experiments, n = 8–12 per group, *p < 0.05; **p < 0.01.