FIGURE 9.

Impact of LDL-R and LRP1 on CTPR9-induced effects on fatty acid metabolism. H9C2 cells (A–C) or neonatal rat cardiomyocytes (D–F) were transfected with siRNA directed against LDL-R, LRP1 or control siRNA (mock). Cells were harvested 48 h after transfection. 24 h after transfection cells were treated with CTRP9 (4 μg/ml) as indicated. (A,D) Representative Western blots of LDL-R and LRP1 are shown. GAPDH served as a loading control. (B,E) Changes in the mRNA expression of CD36 were analyzed by Real-time PCR. (C,F) CD36, LDL-R, and LRP1 protein expression were analyzed by Western Blotting. Representative Western blots and densitometry of protein data are shown. GAPDH served as a loading control. All data are mean ± SEM, 4 independent experiments, n = 8 per group, *p < 0.05, **p < 0.01.