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. 2021 Feb 11;7:591736. doi: 10.3389/fmed.2020.591736

Figure 3.

Figure 3

Depletion of DHX15 induces autophagy associated with suppressing mTORC1. (A) The levels of phosphorylated substrates of the mTORC1 complex in HepG2 cells transiently transfected with DHX15 siRNA (siDHX15) or scrambled control siRNA (siControl) were analyzed by western blotting. Treatment with Torin 1 (250 nM) for 6 h was used as a positive control. Treatment with DMSO was used as a negative control. β-Actin was used as a loading control. *P < 0.05. (B) Data are representative western blots of three independent assays. *P < 0.05, **P < 0.001 (C) HepG2 cells transiently transfected with siDHX15 or siControl were treated with 200 nM rapamycin for 2 h and harvested for western blotting. Treatment with DMSO was used as a negative control. β-Actin was used as a loading control. *P < 0.05. (D) LC3 and SQSTM1/p62 levels in HepG2 cells transiently transfected with siDHX15 or siControl were measured by western blotting. Treatment with Torin 1 (250 nM) for 6 h was used as a positive control. *P < 0.05.