FIGURE 2.

ISL inhibited the production of pro-inflammatory cytokines in BV2 cells induced by AβO. (A–C) BV2 cells were pre-treated with ISL for 2 h and then co-treated with 5 μM AβO for 6 h. The mRNA levels of IL-1β, IL-6, and TNF-α in BV2 cells treated with AβO in the presence or absence of ISL were analyzed by qRT-PCR. (D–F) BV2 cells were pre-treated with ISL for 2 h and then co-treated with 5 μM AβO for 24 h. The production of TNF-α, IL-6, and IL-1β in BV2 cells treated with AβO in the presence or absence of ISL were determined by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001. n = 6 per group.