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. 2021 Feb 11;15:638772. doi: 10.3389/fnins.2021.638772

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Copyright © 2021 Fu and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of Nrf2 in nucleus and cytoplasm. BV2 cells, including those transfected by Nrf2 siRNA, were pre-treated with ISL for 2 h and then co-treated with 5 μM AβO for 24 h. (A,B) Protein expression of cytosolic and nuclear Nrf2 were analyzed by Western blot. (C,D) The translocation of Nrf2 (green) to the nucleus (blue) is shown by confocal microscopic images. Scale bar: 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001. n = 4 per group for Western blot and n = 6 for immunofluorescence staining.