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. 2021 Feb 24;12(2):208. doi: 10.1038/s41419-021-03499-w

Fig. 4. Combination treatment with MSCs and El triggered synergistically differentiation effects in K562 cells.

Fig. 4

A K562 cells were cultured alone or co-cultured with MSCs in the presence or absence of different doses of El (0.5 μM, 1 μM, and 2 μM) for 48 h. The relative gene expression of differentiation genes was assessed by RT-qPCR (n = 3). B Morphological changes in K562 cells that accompanied megakaryocytic differentiation were confirmed by Wright–Giemsa staining of cells in the presence and absence of El (0.5 μM, 1 μM, and 2 μM) for 48 h. Scale bars, 50 μm. C The expression of surface markers CD41 and CD42b of K562 cells was measured by flow cytometric analysis and the mean fluorescence intensity was quantified. D K562 cells were treated with either MSCs or combination with El for 48 h, the expression levels of megakaryocytic differentiation transcription factors were assessed by RT-qPCR (n = 3). Data are represented as the mean ± SEM (*P <0.05, **P <0.01, or ***P <0.001, ns, not significant, one-way ANOVA was used for multiple comparisons). El eltrombopag.