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. 2021 Feb 24;11:4441. doi: 10.1038/s41598-021-81607-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Dose dependent decrease of locomotor activity following e-nicotine exposure. (a) The locomotor activity of 10 flies/group vs time. The number (percentage) of flies that reached a height of 7 cm in 10 s following the exposure to different concentrations of nicotine as compared to the water-exposed controls was recorded (b) Experimental outline, female flies were exposed to 5 mM of volatilized nicotine for 8 times once per an hour, thereafter, the flies were mated with the males of the same age and their offspring were analysed across the timeline. Values are mean ± SEM, one-way ANOVA, **p ≤ 0.01, ****p ≤ 0.0001, n = 3 independent experiments.