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. 2021 Feb 11;9:630353. doi: 10.3389/fcell.2021.630353

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Copyright © 2021 Qiu, Xie, Zhou, Liu, Hu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Derivation of iMSCs from iPSCs. (A) Schematic representation of the in situ genetic correction and targeted gene addition at the hrDNA locus in F8 Inv22 HA iPS cells. (B) Flow cytometry analysis of surface markers in iMSCs derived from iPSCs. HA-iMSCs were derived from F8 Inv22 HA iPS cells. 17-9-iMSCs were derived from F8-corrected iPS cells. T-7-iMSCs were derived from BDDF8-iPS cells. hiMSCs were derived from normal control hiPSCs. (C) Identification of osteogenic, chondrogenic, and adipogenic differential potential of iMSCs.