Figure 3.

PGN induces transient upregulation of the transcription of major pro- and anti-inflammatory markers in BEECs. Subconfluent BEEC monolayers were exposed to different picogram concentrations (10, 100 and 1000 pg ml⁻¹) of PGN at different time points (0, 1, 3, 6, 12, and 24 h). The mRNA expression of major pro- and anti-inflammatory markers was assessed using RT-PCR assay. Data are presented as mean ± SEM of 3 independent experiments using epithelial cells from 3 different uteri (3 wells/treatment/experiment). Asterisks denote a significant variance (*P < 0.05 **P < 0.01, or ***P < 0.001) between the different groups of PGN concentrations (pg/ml) when compared to the control group (0 pg/ml) at each time point.