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Gene expression levels of inflammasome-related genes Nlrp3 (A) and Asc (B), caspase 1 (C) and inflammasome-associated cytokine Il18 (D) in ovaries from 2, 6, 12, and 18-month-old WT, Asc−/−, and Nlrp3−/− mice. n = 3–6 per cohort. Data are presented as mean ± SEM. For each age group, comparisons were made with WT using Student's t-test (A–D) or Mann-Whitney test (D) (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
