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. 2021 Feb 11;11:620038. doi: 10.3389/fphar.2020.620038

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Copyright © 2021 Minutolo, Potestà, Roglia, Cirilli, Iacovelli, Cerva, Fokam, Desideri, Andreoni, Grelli, Colizzi, Muleo and Montesano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of mimic p-miR858b transfection on VAV1 expression and HIV infection. (A) Representative Western Blot of two HIV+ patient of VAV1 expression level with (+) and without (-) mimic p-miR858b. (B) Densitometry histogram of the mean ± S.D. of 15 HIV+ subjects, with and without mimic p-miR858b. (C) Flow cytometry analysis of the percentage of FITC-VAV1 positive cells. (D) Percentage of p24⁺ cells, with and without mimic p-miR858b treatment. (E) Fold change of virus integration level, after transfection with p-miRs and mimic p-miR858b. **p < 0.01. All results derived from at least 15 HIV+ samples. The ANOVA-Bonferroni test was used.