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. 2021 Feb 11;11:603569. doi: 10.3389/fimmu.2020.603569

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Copyright © 2021 Naseraldeen, Michelis, Barhoum, Chezar, Tadmor, Aviv, Shvidel, Litmanovich, Shehadeh, Stemer, Shaoul and Braester

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Separation of the IgG-hexamer samples. (A) Samples of NC (a, b), CLL patients (c–f) and commercial IgG (g, h) were separated and silver stained. Heavy (γ) and light chain are indicated by arrows. Additional proteins besides heavy and light chains are marked in frames. (B) The process of selection of the resulting sequence data included elimination of all the IgG-related sequences, low molecular mass peptides, sequences with a total number of identified peptide sequences (peptide spectrum matches-#PSMs)<30, and sequences with coverage<25.