Figure 2.
Human neutrophil chromosomes distribute randomly to nuclear lobes
(A and B) (A) Heatmap of the -log10(p value) from chromosome lobe colocalization analysis in human blood neutrophil nuclear lobes. The analysis determined if pairs of chromosomes are found colocalized within a lobe at a higher rate than expected by chance (B) Top: Three-dimensional render of chromosomes within a human neutrophil nucleus. Left: Three-dimensional renders of the same neutrophil and chromosomes after five sets of random chromosome shuffling. Right: Heatmap of the -log10(p value) from the chromosome colocalization analysis of five independent sets of random chromosome shuffling.
(C and D) (C) Schematic of the proximity ligation reaction central to the in situ HiC protocol, (D) Proportion of total DNA-DNA interactions detected by in situ HiC and the diffHiC pipeline that occur between chromosomes (transchromosomal interactions) in human immune cells.
(E) Frequency of transchromosomal interactions to the power of 0.25, plotted as a function of summed chromosome length in human neutrophils. Straight line fitted to data with no intercept (y = 8.621e-3x, R2 = 0.9683 and p value <2.2 × 10−16).
(F) Heatmap of the number of transchromosomal interactions by each chromosome to all others in human neutrophils.