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. 2021 Feb 11;11:636965. doi: 10.3389/fonc.2021.636965

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Copyright © 2021 Bai, He, Shi, Wang, Ma, Yang, Dong, Mou and Han

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Linc00963 directly sponges miR-655 in castration-resistant prostate cancer (CRPC) cells. (A) Subcellular localization of Linc00963 in PC-3 cells, detected by FISH; Blue: DAPI, Green: Linc00963-probe; (B, C) Effects of Linc00963 on the expression of miRNAs with putative binding sites for TRIM24 in PC-3 (B) and C4-2 (C) cells; (D) wild type (WT) and mutant (MUT) targeting region of Linc00963 for miR-655; (E) Relative luciferase activity in PC-3 cells co-transfected with WT/MUT Linc00963 plasmid and miR-655 NC/mimics; (F) Enrichment of Linc00963 in the sample pulled down by the Bio-Linc00963-probe and Bio- NC-probe; (G) Enrichment of miR-655 in the sample pulled down by the Bio-Linc00963-probe, Bio-Linc00963-MUT-probe, and Bio- NC-probe; Data are shown as the mean ± S.E. *P < 0.05, **P < 0.01. The representative results of 3 independent experiments are shown.