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. 2021 Feb 11;11:636965. doi: 10.3389/fonc.2021.636965

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Copyright © 2021 Bai, He, Shi, Wang, Ma, Yang, Dong, Mou and Han

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRIM24 is the direct target of miR-655 in castration-resistant prostate cancer (CRPC) cells. (A) Relative luciferase activity in PC-3 cells co-transfected with wild type (WT)/mutant (MUT) TRIM24 plasmid and miR-655 NC/mimics; (B) Putative binding sequence of miR-655 in the 3′-UTR of TRIM24; (C) Effects of miR-655 on TRIM24 protein levels in PC-3 and C4-2 cells after transfection of 50 nM miR-655 inhibitors for 48 h, detected by western blot (WB); (D) Effects of miR-655 on TRIM24 mRNA levels in PC-3 and C4-2 cells after transfection of 50 nM miR-655 mimics for 48 h, detected by RT-QPCR; Data are shown as the mean ± S.E. *P < 0.05.