Figure 4.

ORMDL family-dependent increase in calcium mobilization, β-glucuronidase release and migration in antigen-activated BMMCs. (A) Calcium response in WT (n = 8), O2 KO mice (n = 8), O3 KO mice (n = 5), and O2,3 dKO mice (n = 6). (B) β-glucuronidase release from non-activated and Ag-activated BMMCs isolated from WT, O2 KO, O3 KO and O2,3 dKO mice, n = 5 in all groups (C) Antigen-mediated chemotaxis. IgE-sensitized BMMC derived from WT, O2 KO, O3 KO, and O2,3 dKO mice were examined for their migration through 8 μm-pore-size polycarbonate filters towards antigen (TNP-BSA), n = 3 in all groups. Quantitative data are mean ± s.e.m., calculated from n, which show numbers of biological replicates. P values were determined (in A, B) by two-way ANOVA with Tukey’s post hoc test or by one-way ANOVA with Dunnett’s post hoc test (in C). In A, the range of significant differences (P < 0.05) between O2,3 dKO and WT or O2 KO cells is shown as a blue line, and between ORMDL3 KO and WT or O2 KO cells as a green line. **P < 0.01.