Figure 5.

ORMDL family-dependent changes in phosphorylation of IκB-α upon FcϵRI-mediated activation of BMMCs and cytokine secretion. (A, B) Lysates from nonactivated or activated BMMCs were subjected to immunoblotting analysis with the indicated phospho-specific antibodies. Quantification and statistical evaluation of fold changes in protein phosphorylation were normalized to phosphorylation in non-activated cells and to the amount of the corresponding loading control (SYK, p38) or antibody specific for HPRT was used as a p-IκB-α loading control. Typical results from four to five independent experiments are presented. (C) The levels of IL-4, IL-6, IL-13, and TNF-α released into the supernatant from non-activated (Control) or Ag-activated (Ag) BMMCs were determined by bead-based immunoassay. WT (n = 4), O2 KO mice (n = 3), O3 KO mice (n = 4), and O2,3 dKO mice (n = 4). Quantitative data are mean ± s.e.m., calculated from n, which show numbers of biological replicates. P values in (A–C) were determined by one-way ANOVA with Dunnett’s post hoc test. n. d., Non-determined.