Figure 6.

The role of ORMDL family members in PCA. (A) TNP-specific IgE was injected intradermally into the right ears and PBS alone into the left ears. After 48 h, TNP-BSA was administered intravenously together with Evans blue dye. Mice were killed 1 h later. The examples of excised IgE-positive ears penetrated with Evans blue from WT mice and ORMDL2,3 dKO mice are shown on the left. On the right, quantification of Evans blue extravasation from control ears (PBS) and IgE-positive ears (PBS + IgE). WT mice (n = 13), O2 KO mice (n = 6), O3 KO mice (n = 8), and O2,3 dKO mice (n = 13). (B) The counts of mast cells stained with toluidine blue in the skin cuts; n = 5 in all groups. Quantitative data are mean ± s.e.m., calculated from n, which show numbers of biological replicates. P values in (A, B) were determined by one-way ANOVA with Dunnett’s post hoc test.