Table 2.
Slaughterhouse |
A |
B |
C |
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---|---|---|---|---|---|---|---|---|---|---|---|---|
Samplesa | 1st sampling day |
2nd sampling day |
1st sampling day |
2nd sampling day |
1st sampling day |
2nd sampling day |
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No. | Serotype (N)—pulsotype | No. | Serotype (N)—pulsotype | No. | Serotype—pulsotype | No. | Serotype—pulsotype | No. | Serotype—pulsotype | No. | Serotype (N)—pulsotype | |
Before slaughter | ||||||||||||
Hanging area | ||||||||||||
Three shackles before the hanging area | 1/1 | Infantis—F | ||||||||||
Two wheels and 25 cm transport rail before the hanging area | 1/1 | Infantis—F | ||||||||||
Two wheels and 25 cm transport rail after stunning | 1/1 | Infantis—F | ||||||||||
Scalding tank | ||||||||||||
Three shackles | 1/3 | Infantis—F | 2/3 | Infantis—F’1 | ||||||||
Two wheels and 25 cm transport rail | 2/4 | Infantis—F | 3/3 | Infantis—F | ||||||||
Plucking machine | ||||||||||||
Three shackles | 1/3 | Infantis—F | 1/3 | Infantis—F | 1/3 | Infantis—F′ | ||||||
Two wheels and 25 cm transport rail | 1/3 | Paratyphi B var Java—E | 3/3 | Infantis (2)—F Paratyphi B var Java (1)—E | ||||||||
Plucking fingers −1 element | 2/3 | Infantis—F | 1/3 | Minnesota—B | 1/3 | Rissen—P | 2/3 | Infantis—F′ | ||||
Plastic bands between fingers (400 cm2) | 3/3 | Infantis—F | 1/3 | Typhimurium—V’1 | 1/3 | Infantis—F′ | ||||||
Construction (400 cm2) | 1/3 | Infantis—F | 1/3 | Typhimurium—V | 1/3 | Infantis—F′ | ||||||
Evisceration line | ||||||||||||
Head puller | 1/1 | Infantis—F | ||||||||||
Lung remover—1 element | 1/1 | Infantis—F′ | ||||||||||
Cutting line | ||||||||||||
Two wheels and 25 cm of transport rail | 1/3 | Livingstone—C | ||||||||||
Knife to cleave the thighs in two | 1/1 | Paratyphi B var Java—E | ||||||||||
Samples during slaughter of the first flock | ||||||||||||
Four crates of 6 transport container after use (4 × 400 cm2) | 2/6 | Infantis—F | 2/6 | Infantis—F | 1/6 | Infantis—I | ||||||
Swabs of feathers before scalding | 1/3 | Infantis—F | 2/3 | Infantis—F | 2/3 | Infantis—I/H | ||||||
Swabs of feathers after scalding | 2/3 | Infantis—I | ||||||||||
Feathers from the plucking machine (25g) | 2/3 | Infantis—F | 3/3 | Livingstone—C | 1/3 | Infantis—I | 3/3 | Infantis—I | ||||
25 ml scalding water | 5/6 | Livingstone—C | 2/6 | Typhimurium—V | ||||||||
Neck skins after plucking (25g) | 5/30 | Infantis (1)—F′ Paratyphi B var Java (4)—E |
25/29 | Infantis (21)—F Livingstone (6)—C |
1/30 | Rissen—P | 9/30 | Infantis—I/F′ | ||||
Neck skins after evisceration (25g) | 5/30 | Infantis (4)—F Paratyphi B var Java (2)—E |
23/30 | Infantis(13)—F Livingstone (10)—C |
6/30 | Infantis—I | ||||||
Neck skins after chilling (25g) | 7/30 | Infantis (6)—F Paratyphi B var Java (2)—E |
27/30 | Infantis (20)—F Paratyphi B var Java (1)—E Livingstone (6)—C |
1/30 | Infantis—F°2 | 6/30 | Infantis—I/F | ||||
Thighs (25 g skin) | 1/30 | Paratyphi B var Java—E | 20/30 | Infantis(17)—F Livingstone (4)—C |
3/30 | Infantis (2)—I Enteritidis (1)—M′1 |
N: a: An overview of all samples collected can be seen in Table 1. Only samples that were Salmonella positive on one of the sampling occasions, are shown here.
The symbol “’” after the letter means that up to 2 bands were different between 2 isolates when digested with XbaI.
the symbol “°” after the letter means that the fingerprints were the same when digested with XbaI but a difference of one band was noticed when digested with NotI.