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. 2021 Feb 19;14(4):101039. doi: 10.1016/j.tranon.2021.101039

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — NSE promoted migration and invasion in SCLC cells. (A) The protein expressions of NSE in different SCLC cell lines (H446, H69, H209) were detected by western blotting. (B–D) The efficiency of NSE overexpression or knockdown was verified by western blotting (B and C) and qRT-PCR (D). (E) migration ability of the H446 cells overexpressing NSE or control cells was detected by wound-healing assay (Scale bar: 100 um). (F) Transwell assay was used to analyze the migration and invasion capabilities of NSE-overexpressed H446 cells (Scale bar: 50 um). (G) The effect of NSE knockdown on the migration capability of H69 cells was analyzed by Transwell assay. The results are expressed as the mean ± SD of three independent experiments. *p < 0.05 using the two-sided Student's t-test.